Composite

Part:BBa_K199062:Design

Designed by: Olivia Ho-Shing   Group: iGEM09_MoWestern_Davidson   (2009-08-06)


pBad-RBS-RFP-pBad-RBS-Tet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 998
    Illegal NheI site found at 1176
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 938
    Illegal BamHI site found at 1322
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1348
    Illegal NgoMIV site found at 1716
    Illegal NgoMIV site found at 1876
    Illegal AgeI site found at 714
    Illegal AgeI site found at 826
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that this part in a drug resistance plasmid subjects the cell to two drug resistances, including tetracycline. This may tend to make cells less hardy and grow slower.


Source

From the BioBrick Parts Registry.

References